Review



fc blocking buffer  (Miltenyi Biotec)


Bioz Verified Symbol Miltenyi Biotec is a verified supplier
Bioz Manufacturer Symbol Miltenyi Biotec manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97

    Structured Review

    Miltenyi Biotec fc blocking buffer
    Fc Blocking Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1283 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fc+blocking+buffer/FcR+Blocking+Reagent%2C+mouse/pm41913646-422-20-22
    Average 97 stars, based on 1283 article reviews
    fc blocking buffer - by Bioz Stars, 2026-11
    97/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Extrahepatic Gene Editing In Vivo Using Organic Solvent-Free Lipid Nanoparticles.
    Article Snippet: Analysis was conducted with Kaluza V 2.2.1 (Beckmann Coulter). .. To additionally identify the amount of CD45+ cells in the spleen samples additional cell suspensions were incubated with 50 μL Fc-blocking buffer (Miltenyi, Cat No. 130-092-575) at 4◦C for 10 min. .. The blocked samples were then stained with PerCP-CD45 (Biolegend, Clone: 30-F11, Cat No. 103 130) at 4◦C in the dark for 30 min. After one wash with staining buffer at 500 rcf for 5 min and resuspended in 200 μL stain buffer.

    Staining:

    Article Title: Combination therapy with 2-(4-chlorophenyl)-N-((2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)methyl)-2,2-difluoroacetamide
    Article Snippet: Antibodies used for immunoblot: mouse anti-human GSPT-1 mAb conjugated with AL647 (clone number GSPT1-164-17G9-G6, Celgene, San Diego, CA), IL-1β (R&D Systems, Cat No. AF-201-NA), cleaved caspase 3 (Cell Signaling, Cat No. 9664S), alpha-tubulin (Cell Signaling, Cat No. 3873S), cleaved Caspase-8 (Cell Signaling, Cat No. 9748S), Caspase-1 (Cell Signaling, Cat No. 3866S), HA tag (Cell Signaling, Cat No. 3724), phosphor-eIF2a (Cell Signaling, Cat No. 9721), total eIF2a (Cell Signaling, Cat No. 9722), ATF4 (Cell Signaling, Cat No. 11815S), CHOP (Abcam, Cat No. 11419), GAPDH (Cell Signaling, Cat No. 5174), rabbit anti-mouse 800 antibody (LI-COR Biosciences), rabbit anti-mouse 680 antibody (LI-COR Biosciences), mouse anti-rabbit 680 antibody (LI-COR Biosciences), mouse anti-rabbit 800 antibody (LI-COR Biosciences) Flow Cytometry Analysis Sample processing for flow cytometry was performed in 96 well plates. .. One million cells per well were washed with 300 μL ice cold staining buffer (BD, Cat No. 554723) and blocked with 150 μL Fc blocking buffer (Miltenyi, Cat No. 130-059-901) for 10 minutes at room temperature. .. After blocking, cells were washed once with perm/wash buffer (BD, Cat No. 554723) and stained with 100 μL primary antibody diluted in perm/wash buffer for 20 min on ice in the dark.

    Blocking Assay:

    Article Title: Combination therapy with 2-(4-chlorophenyl)-N-((2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)methyl)-2,2-difluoroacetamide
    Article Snippet: Antibodies used for immunoblot: mouse anti-human GSPT-1 mAb conjugated with AL647 (clone number GSPT1-164-17G9-G6, Celgene, San Diego, CA), IL-1β (R&D Systems, Cat No. AF-201-NA), cleaved caspase 3 (Cell Signaling, Cat No. 9664S), alpha-tubulin (Cell Signaling, Cat No. 3873S), cleaved Caspase-8 (Cell Signaling, Cat No. 9748S), Caspase-1 (Cell Signaling, Cat No. 3866S), HA tag (Cell Signaling, Cat No. 3724), phosphor-eIF2a (Cell Signaling, Cat No. 9721), total eIF2a (Cell Signaling, Cat No. 9722), ATF4 (Cell Signaling, Cat No. 11815S), CHOP (Abcam, Cat No. 11419), GAPDH (Cell Signaling, Cat No. 5174), rabbit anti-mouse 800 antibody (LI-COR Biosciences), rabbit anti-mouse 680 antibody (LI-COR Biosciences), mouse anti-rabbit 680 antibody (LI-COR Biosciences), mouse anti-rabbit 800 antibody (LI-COR Biosciences) Flow Cytometry Analysis Sample processing for flow cytometry was performed in 96 well plates. .. One million cells per well were washed with 300 μL ice cold staining buffer (BD, Cat No. 554723) and blocked with 150 μL Fc blocking buffer (Miltenyi, Cat No. 130-059-901) for 10 minutes at room temperature. .. After blocking, cells were washed once with perm/wash buffer (BD, Cat No. 554723) and stained with 100 μL primary antibody diluted in perm/wash buffer for 20 min on ice in the dark.



    Similar Products

    97
    Miltenyi Biotec fc blocking buffer
    Fc Blocking Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fc+blocking+buffer/FcR+Blocking+Reagent%2C+mouse/pm41913646-422-20-22
    Average 97 stars, based on 1 article reviews
    fc blocking buffer - by Bioz Stars, 2026-11
    97/100 stars
      Buy from Supplier

    93
    Bio-Rad fc block buffer
    Fc Block Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fc+blocking+buffer/Rat+anti+Mouse+CD16%2FCD32/pm41663544-252-16-18
    Average 93 stars, based on 1 article reviews
    fc block buffer - by Bioz Stars, 2026-11
    93/100 stars
      Buy from Supplier

    96
    Bio X Cell fc receptor blocking buffer
    Fc Receptor Blocking Buffer, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fc+blocking+buffer/InVivoMAb+anti-mouse+CD16+CD32/pm41611675-309-24-32
    Average 96 stars, based on 1 article reviews
    fc receptor blocking buffer - by Bioz Stars, 2026-11
    96/100 stars
      Buy from Supplier

    90
    Becton Dickinson blocking buffer becton dickinson human fc block
    Blocking Buffer Becton Dickinson Human Fc Block, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fc+blocking+buffer/blocking+buffer+becton+dickinson+human+fc+block/pmc11965450__41598_2025_94295_MOESM2_ESM-17-29-32
    Average 90 stars, based on 1 article reviews
    blocking buffer becton dickinson human fc block - by Bioz Stars, 2026-11
    90/100 stars
      Buy from Supplier

    90
    Bio X Cell fc-block (1:100 in facs buffer)
    Fc Block (1:100 In Facs Buffer), supplied by Bio X Cell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fc+blocking+buffer/anti+cd16+32/bio_rxiv__2024__12__20__629639-174-30-35
    Average 90 stars, based on 1 article reviews
    fc-block (1:100 in facs buffer) - by Bioz Stars, 2026-11
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher fc-blocking buffer (chromepure mouse igg
    Fc Blocking Buffer (Chromepure Mouse Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fc+blocking+buffer/pm39857770-65-36-41
    Average 90 stars, based on 1 article reviews
    fc-blocking buffer (chromepure mouse igg - by Bioz Stars, 2026-11
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher fc-blocking buffer
    Fc Blocking Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fc+blocking+buffer/pmc11761615-78-36-41
    Average 90 stars, based on 1 article reviews
    fc-blocking buffer - by Bioz Stars, 2026-11
    90/100 stars
      Buy from Supplier

    Image Search Results